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使用脱脂牛奶絮凝和超滤法浓缩环境水和废水样品中的病毒颗粒

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Concentration of Virus Particles from Environmental Water and Wastewater Samples Using Skimmed Milk Flocculation and Ultrafiltration

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使用脱脂牛奶絮凝和超滤法浓缩环境水和废水样品中的病毒颗粒

DOI:

10.3791/65058-v

•

10:53 min

•

March 17, 2023

•

Kadir Yanaç, Jhannelle Francis, Jocelyn Zambrano-Alvarado, Qiuyan Yuan, Miguel Uyaguari-Díaz

¹Department of Civil Engineering, Price Faculty of Engineering,University of Manitoba, ²Department of Microbiology, Faculty of Science,University of Manitoba

Capítulos

00:04Introduction
01:23Comparison of Serial Ultrafiltration and Skimmed Milk Flocculation to Concentrate Viruses in Wastewater Samples
04:53Filtration and Concentration of DNA and RNA Viruses for Water‐Based Epidemiology
07:28Direct Precipitation and Concentration of Microbial Fractions for Water‐Based Epidemiology
08:54Results: Effectiveness of Ultrafiltration and Skimmed Milk Flocculation for RNA Viruses with Different Sample Types
10:10Conclusion

Summary

Tadução automática

环境水和废水样品中的病毒浓度是一项具有挑战性的任务，主要用于病毒的鉴定和定量。虽然已经开发和测试了几种病毒浓缩方法，但我们在这里展示了超滤和脱脂牛奶絮凝对不同样品类型的RNA病毒的有效性。

Tags

Keywords: Virus Concentration Wastewater Epidemiology Skimmed Milk Flocculation Ultrafiltration Metagenomics PCR Wastewater Samples Armored RNA PH Adjustment Centrifugation

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