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使用脱脂牛奶絮凝和超滤法浓缩环境水和废水样品中的病毒颗粒
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Concentration of Virus Particles from Environmental Water and Wastewater Samples Using Skimmed Milk Flocculation and Ultrafiltration
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使用脱脂牛奶絮凝和超滤法浓缩环境水和废水样品中的病毒颗粒

1,038 Views

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10:53 min

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March 17, 2023

DOI:

10.3791/65058-v

DOI:
10.3791/65058-v

•

10:53 min
March 17, 2023

•

46 Views
, , , ,
1Department of Civil Engineering, Price Faculty of Engineering,University of Manitoba, 2Department of Microbiology, Faculty of Science,University of Manitoba

Summary

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环境水和废水样品中的病毒浓度是一项具有挑战性的任务，主要用于病毒的鉴定和定量。虽然已经开发和测试了几种病毒浓缩方法，但我们在这里展示了超滤和脱脂牛奶絮凝对不同样品类型的RNA病毒的有效性。

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Virus ConcentrationWastewater EpidemiologySkimmed Milk FlocculationUltrafiltrationMetagenomicsPCRWastewater SamplesArmored RNAPH AdjustmentCentrifugation

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Cite this Article

Yanaç, K., Francis, J., Zambrano-Alvarado, J., Yuan, Q., Uyaguari-Díaz, M. Concentration of Virus Particles from Environmental Water and Wastewater Samples Using Skimmed Milk Flocculation and Ultrafiltration. J. Vis. Exp. (193), e65058, doi:10.3791/65058 (2023).

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