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Concentration of Virus Particles from Environmental Water and Wastewater Samples Using Skimmed Milk Flocculation and Ultrafiltration
 

Concentration of Virus Particles from Environmental Water and Wastewater Samples Using Skimmed Milk Flocculation and Ultrafiltration

Article DOI: 10.3791/65058-v • 10:53 min • March 17th, 2023
March 17th, 2023 •
1, 2, 2, 1, 2
1Department of Civil Engineering, Price Faculty of Engineering, University of Manitoba, 2Department of Microbiology, Faculty of Science, University of Manitoba

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Virus concentration from environmental water and wastewater samples is a challenging task, carried out primarily for the identification and quantification of viruses. While several virus concentration methods have been developed and tested, we demonstrate here the effectiveness of ultrafiltration and skimmed milk flocculation for RNA viruses with different sample types.

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Virus Particles Environmental Water Wastewater Samples Skimmed Milk Flocculation Ultrafiltration Water-based Epidemiology Outbreaks Concentrate Microbes Metagenomics Targeted PCR Studies Nucleic Acid Extraction Commercial Extraction Kits In-house Protocol Large Volumes Small Volumes Milli-Q Water Control Kadir Yanac Jhannelle Francis Jocelyn Zambrano-Alvarado Graduate Students Miguel Uyaguari's Laboratory 24-hour Flow Proportional Composite Raw Wastewater Samples Wastewater Treatment Plant Spike Virus Particles
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