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Biologia
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Culture of Piglet Intestinal 3D Organoids from Cryopreserved Epithelial Crypts and Establishment of Cell Monolayers
JoVE Journal
Biologia
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JoVE Journal Biologia
Culture of Piglet Intestinal 3D Organoids from Cryopreserved Epithelial Crypts and Establishment of Cell Monolayers

Culture of Piglet Intestinal 3D Organoids from Cryopreserved Epithelial Crypts and Establishment of Cell Monolayers

DOI:
10.3791/64917-v

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08:19 min

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February 10, 2023

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, , , , , ,
1GenPhySE,Université de Toulouse, INRAE, ENVT, 2Lallemand Animal Nutrition, 3Institut NuMeCan, INRAE, INSERM,Université de Rennes, 4FG 16: Mycotic and Parasitic Agents and Mycobacteria,Robert Koch-Institute

Capítulos

  • 00:04Introduction
  • 00:50Establishment of Piglet Intestinal 3D Organoids from Frozen Epithelial Crypts
  • 01:52Passage of Piglet Intestinal 3D Organoids Derived from Frozen Crypts
  • 03:54Culture of Cell Monolayers Derived from 3D Organoids
  • 06:17Results: Characteristics of Cell Monolayers Derived from Pig Intestinal 3D Organoids
  • 07:37Conclusion

Summary

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Here, we describe a protocol to culture pig intestinal 3D organoids from cryopreserved epithelial crypts. We also describe a method to establish cell monolayers derived from 3D organoids, allowing access to the apical side of epithelial cells.

Tags

Pig Intestinal EpitheliumIntestinal Organoids3D OrganoidsCryopreserved CryptsEpithelial Crypts2D MonolayersNutrient TransportBarrier FunctionHost-microbe InteractionsJejunumDuodenumIleumColonExtracellular MatrixECMCulture MediumPassagingDissociationDMEMRock Inhibitor

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