/
/
Infection of Primary Nasal Epithelial Cells Grown at an Air-Liquid Interface to Characterize Human Coronavirus-Host Interactions
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Infection of Primary Nasal Epithelial Cells Grown at an Air-Liquid Interface to Characterize Human Coronavirus-Host Interactions
972 Views
•
09:02 min
•
September 22, 2023
DOI:
1Department of Microbiology,University of Pennsylvania, 2Penn Center for Research on Coronaviruses and Other Emerging Pathogens, Perelman School of Medicine,University of Pennsylvania, 3Otorhinolaryngology-Head and Neck Surgery,University of Pennsylvania, 4Corporal Michael J. Crescenz VA Medical Center
Summary
Automatically generated
The nasal epithelium is the primary barrier site encountered by all respiratory pathogens. Here, we outline methods to use primary nasal epithelial cells grown as air-liquid interface (ALI) cultures to characterize human coronavirus-host interactions in a physiologically relevant system.
Read Article
Cite this Article
Otter, C. J., Fausto, A., Tan, L. H., Weiss, S. R., Cohen, N. A. Infection of Primary Nasal Epithelial Cells Grown at an Air-Liquid Interface to Characterize Human Coronavirus-Host Interactions. J. Vis. Exp. (199), e64868, doi:10.3791/64868 (2023).
Copy