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Primer Rhesus Macaque B Hücrelerinin Gen Düzenlemesi
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February 10, 2023
DOI:
1Laboratory of Molecular Immunology,The Rockefeller University, 2Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases,National Institutes of Health, 3Laboratory of Retrovirology,The Rockefeller University, 4Howard Hughes Medical Institute,The Rockefeller University, 5Laboratory of Applied Virology and Precision Medicine,King Abdullah University of Science and Technology (KAUST), 6Vaccine and Immunotherapy Center,Wistar Institute
Summary
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B hücre tedavilerinin incelenmesi için CRISPR / Cas9 ve rekombinant adeno ilişkili virüs serotip 6 kullanarak primer rhesus makak B hücrelerinin kültürlenmesi ve gen düzenlenmesi için bir yöntem sunuyoruz.
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Hartweger, H., Gautam, R., Nishimura, Y., Schmidt, F., Yao, K., Escolano, A., Jankovic, M., Martin, M. A., Nussenzweig, M. C. Gene Editing of Primary Rhesus Macaque B Cells. J. Vis. Exp. (192), e64858, doi:10.3791/64858 (2023).
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