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February 10, 2023
DOI:
1Laboratory of Molecular Immunology,The Rockefeller University, 2Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases,National Institutes of Health, 3Laboratory of Retrovirology,The Rockefeller University, 4Howard Hughes Medical Institute,The Rockefeller University, 5Laboratory of Applied Virology and Precision Medicine,King Abdullah University of Science and Technology (KAUST), 6Vaccine and Immunotherapy Center,Wistar Institute
Summary
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B세포 치료제 연구를 위해 CRISPR/Cas9 및 재조합 아데노 관련 바이러스 혈청형 6을 이용하여 일차 붉은털원숭이 B세포를 배양하고 유전자를 편집하는 방법을 제시합니다.
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Hartweger, H., Gautam, R., Nishimura, Y., Schmidt, F., Yao, K., Escolano, A., Jankovic, M., Martin, M. A., Nussenzweig, M. C. Gene Editing of Primary Rhesus Macaque B Cells. J. Vis. Exp. (192), e64858, doi:10.3791/64858 (2023).
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