监测斑马鱼幼虫的靶向信号反应 - Z-REX 揭示亲电药物和代谢物的精确机制

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Monitoring On-Target Signaling Responses in Larval Zebrafish – Z-REX Unmasks Precise Mechanisms of Electrophilic Drugs and Metabolites

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监测斑马鱼幼虫的靶向信号反应 - Z-REX 揭示亲电药物和代谢物的精确机制

DOI:

10.3791/64846-v

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05:28 min

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June 02, 2023

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Kuan-Ting Huang, Phillippe Ly, Jesse R. Poganik, Saba Parvez, Marcus J. C. Long, Yimon Aye

¹Swiss Federal Institute of Technology Lausanne (EPFL), ²Division of Genetics, Department of Medicine, Brigham and Women’s Hospital,Harvard Medical School, ³Department of Pharmacology and Toxicology, College of Pharmacy,University of Utah, ⁴University of Lausanne (UNIL)

Capítulos

00:04Introduction
01:02Fish Embryo Microinjection
01:45Z‐REX Treatment
02:31Downstream Assay
03:13Results: Analysis of Treated and Untreated Embryos
04:28Conclusion

Summary

Tadução automática

斑马鱼靶向反应性亲电试剂和氧化剂（Z-REX）是一种基于化学生物学的方法，用于研究反应性小分子信号传导。该技术可应用于不同发育阶段的活鱼。在这里，我们将斑马鱼的标准测定与Z-REX相结合以进行信号通路分析。

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