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Differensiering av humane pluripotente stamceller i insulinproduserende øyklynger
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Differentiation of Human Pluripotent Stem Cells into Insulin-Producing Islet Clusters

Differensiering av humane pluripotente stamceller i insulinproduserende øyklynger

1,523 Views

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08:41 min

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June 23, 2023

DOI:

10.3791/64840-v

DOI:
10.3791/64840-v

•

08:41 min
June 23, 2023

•

7 Views
, , , , , ,
1Department of Cellular & Physiological Sciences, Life Science Institute,University of British Columbia, 2School of Biomedical Engineering,University of British Columbia, 3STEMCELL Technologies Inc, 4Department of Surgery,University of British Columbia

Tags

Human Pluripotent Stem CellsInsulin-producing Islet ClustersStatic Suspension CulturePancreatic ProgenitorsIslet-like ClustersHESC-qualified MatrigelCell DissociationStem Cell CultureStage 1A MediumStage 1B MediumStage 2A MediumStage 2B MediumStage 3 MediumStage 4 MediumAnti-adherence Rinsing SolutionMicrowell Plate

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Zhao, J., Liang, S., Braam, M. J. S., Baker, R. K., Iworima, D. G., Quiskamp, N., Kieffer, T. J. Differentiation of Human Pluripotent Stem Cells into Insulin-Producing Islet Clusters. J. Vis. Exp. (196), e64840, doi:10.3791/64840 (2023).

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