É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
使用改良的酶联免疫吸附测定法检测和定量人血浆中的降钙素基因相关肽 （CGRP）
2,181 Views
•
07:14 min
•
June 16, 2023
DOI:
1Department of Otolaryngology-Head & Neck Surgery,Johns Hopkins University School of Medicine, 2Virginia Commonwealth University School of Medicine, 3Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center,Johns Hopkins University School of Medicine, 4Department of Otolaryngology-Head and Neck Surgery,University of Michigan Medical School, 5Department of Otolaryngology-Head and Neck Surgery,The Ohio State University College of Medicine, 6ICTR Clinical Research Core Laboratory,Johns Hopkins University School of Medicine
Summary
Automatically generated
关于人血浆中降钙素基因相关肽（CGRP）浓度的已发表数据不一致。这些不一致可能是由于缺乏标准化的，经过验证的方法来量化这种神经肽。在这里，我们描述了一种经过验证的酶联免疫吸附测定（ELISA）方案，用于纯化和定量人血浆中的CGRP。
Read Article
Cite this Article
Krishnan, P. S., Zamuner, F. T., Jenks, C. M., Xie, J. Y., Zhang, L., Lehar, M., Fedarko, N. S., Brait, M., Carey, J. P. Detection and Quantification of Calcitonin Gene-Related Peptide (CGRP) in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay. J. Vis. Exp. (196), e64775, doi:10.3791/64775 (2023).
Copy