将IL-33刺激的巨噬细胞过继转移到博来霉素诱导的小鼠模型中，以研究其对体内特发性肺纤维化的影响

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Adoptive Transfer of IL-33-Stimulated Macrophages into Bleomycin-Induced Mouse Models to Study Their Effect on Idiopathic Pulmonary Fibrosis In Vivo

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将IL-33刺激的巨噬细胞过继转移到博来霉素诱导的小鼠模型中，以研究其对体内特发性肺纤维化的影响

DOI:

10.3791/64742-v

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06:29 min

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May 05, 2023

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Xiaorun Zhai*¹, Jiao Li*¹, Yunjuan Nie

¹Department of Basic Medicine, Wuxi School of Medicine,Jiangnan University

Capítulos

00:04Introduction
00:39Preparation of Recipient Mouse
01:13Isolation and Culture of Interstitial Macrophages (IMs)
03:13Adoptive Transfer of IMs into the Lung Alveoli
05:12Results: Effect of IL‐33‐Stimulated Macrophages on Idiopathic Pulmonary Fibrosis
05:59Conclusion

Summary

Tadução automática

该协议描述了肺间质巨噬细胞（IMs）的分离及其在小鼠模型中IL-33刺激肺泡后的过继转移，这可以促进特发性肺纤维化（IPF）的体内研究。

将IL-33刺激的巨噬细胞过继转移到博来霉素诱导的小鼠模型中，以研究其对体内特发性肺纤维化的影响

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