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A Human Bone Marrow 3D Model to Investigate the Dynamics and Interactions Between Resident Cells in Physiological or Tumoral Contexts
 

A Human Bone Marrow 3D Model to Investigate the Dynamics and Interactions Between Resident Cells in Physiological or Tumoral Contexts

Article DOI: 10.3791/64736-v • 09:07 min • December 16th, 2022
December 16th, 2022 •
1,2,3, 1,2,3,4, 1,2,3, 1,2,3, 1,2,3,4, 1,2,3, 1,2,3, 5, 1,2,3, 1,2,3, 1,2,3
1Centre de Recherche en Cancérologie de Lyon, 2Centre de Recherche en Cancérologie de Lyon, 3Université de Lyon, 4Centre Léon Bérard, 5Department of Experimental and Clinical Biomedical Sciences Mario Serio, Università degli Studi di Firenze

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Here, we describe an easy-to-implement, standardized, microphysiological system that reflects the complexity of the human bone marrow's in vivo structure, providing a pertinent model to finely study a broad range of normal and pathological events.

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Human Bone Marrow 3D Model Dynamics Interactions Resident Cells Physiological Context Tumoral Context Standardized System Cell Lines Primary Cell Populations Well-organized Structure Cell Harvest In Situ Cell Localization Imaging 3D Structure Dissociation Molecular Studies Functional Studies BCP Beads Polycarbonate Membrane Six-well Plate PBS Washing Step FBS Blocking Solution
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