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Concanavalin A-Based Sedimentation Assay to Measure Substrate Binding of Glucan Phosphatases
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Concanavalin A-Based Sedimentation Assay to Measure Substrate Binding of Glucan Phosphatases
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December 23, 2022
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This method describes a lectin-based in vitro sedimentation assay to quantify the binding affinity of glucan phosphatase and amylopectin. This co-sedimentation assay is reliable for measuring glucan phosphatase substrate binding and can be applied to various solubilized glucan substrates.
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Wolpaw, E. M., Frenett, M. L., Mak, C. A., Zwanger, S. M., Raththagala, M. Concanavalin A-Based Sedimentation Assay to Measure Substrate Binding of Glucan Phosphatases. J. Vis. Exp. (190), e64700, doi:10.3791/64700 (2022).
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