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Biologia do Desenvolvimento
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Generation of Natural Killer Cells from Human Expanded Potential Stem Cells
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Generation of Natural Killer Cells from Human Expanded Potential Stem Cells

Generation of Natural Killer Cells from Human Expanded Potential Stem Cells

DOI:
10.3791/64608-v

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06:53 min

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January 13, 2023

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, , , ,
1Major in Molecular Biology and Biotechnology, School of Biological Sciences,The University of Hong Kong, 2School of Biomedical Sciences,The University of Hong Kong, 3Centre for Translational Stem Cell Biology

Capítulos

  • 00:04Introduction
  • 00:33Generation of 3D Organoid Structures From hEPSCs
  • 02:02Mesoderm and Hemato-Endothelial Patterning of the Embryoid Body
  • 03:07NK Cell Differentiation
  • 05:05Results: Analysis of In Vitro Differentiation (IVD) Products
  • 06:29Conclusion

Summary

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The present protocol shows how to differentiate CD3−/CD45+CD56+ cells with mild cytotoxicity from human expanded potential stem cells (hEPSCs) under both 3D and 2D culture conditions. This allows for routine phenotypical validation without the destruction of the complex microenvironment.

Tags

Keywords: Natural Killer CellsHuman Expanded Potential Stem CellsCancer ImmunotherapyEmbryoid BodiesMesoderm PatterningHematoendothelial SpecificationDifferentiationCell CultureCell SuspensionCell CountingCell Harvesting

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