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Primer İnsan Retinal Pigment Epitel Hücrelerinde Yüksek Çözünürlüklü Respirometri Kullanılarak Biyoenerjetik Biyoenerjetik Analizinin Gerçek Zamanlı Analizi
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Biologia
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JoVE Journal Biologia
Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution Respirometry
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Primer İnsan Retinal Pigment Epitel Hücrelerinde Yüksek Çözünürlüklü Respirometri Kullanılarak Biyoenerjetik Biyoenerjetik Analizinin Gerçek Zamanlı Analizi

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09:16 min

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February 03, 2023

DOI:

10.3791/64572-v

DOI:
10.3791/64572-v

•

09:16 min
February 03, 2023

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7 Views
, , , , ,
1Schepens Eye Research Institute of Mass. Eye and Ear, 2Department of Ophthalmology,Harvard Medical School

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Real-time BioenergeticsPrimary Human Retinal Pigment Epithelial CellsHigh-resolution RespirometryOxphosGlycolysisAMDCell CultureRespirometry InstrumentMito Stress Test Assay

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Fitch, T. C., Frank, S. I., Li, Y. K., Saint-Geniez, M., Kim, L. A., Shu, D. Y. Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution Respirometry. J. Vis. Exp. (192), e64572, doi:10.3791/64572 (2023).

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