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Compost Microcosms as Microbially Diverse, Natural-like Environments for Microbiome Research in Caenorhabditis elegans
 

Compost Microcosms as Microbially Diverse, Natural-like Environments for Microbiome Research in Caenorhabditis elegans

Article DOI: 10.3791/64393-v • 07:19 min • September 13th, 2022
September 13th, 2022 •
1, 1, 1
1Department of Integrative Biology, University of California, Berkeley

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Compost microcosms bring the microbial diversity found in nature into the laboratory to facilitate microbiome research in Caenorhabditis elegans. Provided here are protocols for setting up microcosm experiments, with the experiments demonstrating the ability to modulate environmental microbial diversity to explore the relationships between environmental microbial diversity and worm gut microbiome composition.

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Compost Microcosms Microbiome Research Caenorhabditis Elegans Host-microbiome Interactions Natural-like Context Synthetic Microbial Communities Compost Or Garden Soil Kitchen Plastic Container Chopped Produce M9 Medium Glass Beaker Tinfoil Centrifuge Bacterial Extract
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