Journal
/
Bioengenharia
/
体外 使用纯化的重组 果蝇 半胱天冬酶进行底物筛选的切割测定
JoVE Journal
Bioengenharia
This content is Free Access.
JoVE Journal Bioengenharia
In Vitro Cleavage Assays using Purified Recombinant Drosophila Caspases for Substrate Screening
Please note that all translations are automatically generated. Click here for the English version.

体外 使用纯化的重组 果蝇 半胱天冬酶进行底物筛选的切割测定

1,185 Views

•

08:16 min

•

October 06, 2022

DOI:

10.3791/64392-v

DOI:
10.3791/64392-v

•

08:16 min
October 06, 2022

•

4 Views
,
1Department of Molecular, Cell and Cancer Biology,UMass Chan Medical School

Tags

In Vitro Cleavage AssayCaspasesDrosophilaDroncRecombinant ProteinsSubstrate ScreeningCell LysisNi-NTA AgarosePurificationEnzymatic Activity

Read Article

Cite this Article

Yarikipati, P., Bergmann, A. In Vitro Cleavage Assays using Purified Recombinant Drosophila Caspases for Substrate Screening. J. Vis. Exp. (188), e64392, doi:10.3791/64392 (2022).

Download .ris file

Copy

Share Video

.

Copy