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聚合物拥挤脂质膜上的单分子扩散和组装
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Bioquímica
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JoVE Journal Bioquímica
Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes
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聚合物拥挤脂质膜上的单分子扩散和组装

DOI:
10.3791/64243-v

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10:43 min

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July 19, 2022

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, , , , ,
1Department of Chemical Engineering,Indian Institute of Science, 2Holonyak Micro & Nanotechnology Lab,University of Illinois, Urbana-Champaign, 3Center for BioSystems Science and Engineering,Indian Institute of Science

Capítulos

  • 00:04Introduction
  • 00:53Assembly of the Microfluidic Chamber
  • 01:41Making Supported Bilayers on Glass Substrate by Vesicle Fusion
  • 04:22Microscope Setup and Single-Particle Imaging Measurements
  • 07:26Image Acquisition for Counting Subunits in a Protein Assembly
  • 08:40Results: Analyzing the Binding of Cytolysin A (ClyA) Protein and Mobility of DNA Tracer
  • 10:10Conclusion

Summary

Tadução automática

Tadução automática

在这里，提出了一种使用单分子全内反射荧光（smTIRF）显微镜执行和分析单分子在人工拥挤脂质膜上的结合，迁移率和组装的协议。

Tags

Single-molecule ImagingPolymer-crowded Lipid MembranesSupported Lipid BilayersMembrane Biomolecular ReactionsDiffusionAssemblyPOPCDOPE-PEG(2000)Small Unilamellar VesiclesSonicationMicrofluidic Imaging Chambers

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