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אין ויוו הדמיית סידן בשדה רחב ושני פוטונים מעכבר באמצעות חלון גולגולתי גדול
JoVE Journal
Neurociência
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JoVE Journal Neurociência
In Vivo Wide-Field and Two-Photon Calcium Imaging from a Mouse Using a Large Cranial Window
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אין ויוו הדמיית סידן בשדה רחב ושני פוטונים מעכבר באמצעות חלון גולגולתי גדול

4,935 Views

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06:45 min

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August 04, 2022

DOI:

10.3791/64224-v

DOI:
10.3791/64224-v

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06:45 min
August 04, 2022

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1Department of Neurophysiology, Faculty of Medicine,University of Yamanashi, 2Department of Neuropharmacology, Faculty of Medicine,University of Yamanashi, 3Yamanashi GLIA center, Interdisciplinary Graduate School of Medicine,University of Yamanashi, 4Department of Neurochemistry, Graduate School of Medicine,The University of Tokyo

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Cranial WindowWide-field Calcium ImagingTwo-photon Calcium ImagingMouseNeural ActivityGlial ActivityComportamentoMacroscopicMicroscopicPVDC WrapACSFBiological AdhesiveSilicone ElastomerCover GlassFibroinAAV

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Manita, S., Shigetomi, E., Bito, H., Koizumi, S., Kitamura, K. In Vivo Wide-Field and Two-Photon Calcium Imaging from a Mouse Using a Large Cranial Window. J. Vis. Exp. (186), e64224, doi:10.3791/64224 (2022).

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