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体内 使用大颅窗的小鼠宽场和双光子钙成像
JoVE Journal
Neurociência
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JoVE Journal Neurociência
In Vivo Wide-Field and Two-Photon Calcium Imaging from a Mouse Using a Large Cranial Window
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体内 使用大颅窗的小鼠宽场和双光子钙成像

5,205 Views

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06:45 min

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August 04, 2022

DOI:

10.3791/64224-v

DOI:
10.3791/64224-v

•

06:45 min
August 04, 2022

•

29 Views
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1Department of Neurophysiology, Faculty of Medicine,University of Yamanashi, 2Department of Neuropharmacology, Faculty of Medicine,University of Yamanashi, 3Yamanashi GLIA center, Interdisciplinary Graduate School of Medicine,University of Yamanashi, 4Department of Neurochemistry, Graduate School of Medicine,The University of Tokyo

Summary

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本协议描述了使用食品包装，透明硅胶和盖玻片制作大型（6 x 3 mm2）颅窗。该颅窗允许在同一只小鼠中进行 体内 宽场和双光子钙成像实验。

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Cite this Article

Manita, S., Shigetomi, E., Bito, H., Koizumi, S., Kitamura, K. In Vivo Wide-Field and Two-Photon Calcium Imaging from a Mouse Using a Large Cranial Window. J. Vis. Exp. (186), e64224, doi:10.3791/64224 (2022).

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