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Separación de subpoblaciones de células inmunitarias en muestras de sangre periférica de niños con mononucleosis infecciosa
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1Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children’s Hospital,Capital Medical University, National Center for Children’s Health, 2Research Unit of Critical Infection in Children, 2019RU016,Chinese Academy of Medical Sciences
Capítulos
- 00:05Introduction
- 00:36Isolation of CD14+ Monocytes from PBMCs Using CD14 Microbeads
- 02:38Fluorescently Labeled Antibody Staining
- 03:52Flow Cytometry Parameter Setting
- 04:32Gating
- 06:18Cell Sorting and Collection via Flow Cytometry
- 07:00Results: Comparison of Immune Cell Subpopulations from Patients with Infectious Mononucleosis, Healthy Epstein Barr Virus Carriers, and Uninfected Samples
- 08:14Conclusion
Describimos un método que combina perlas inmunomagnéticas y clasificación celular activada por fluorescencia para aislar y analizar subpoblaciones de células inmunes definidas de células mononucleares de sangre periférica (monocitos, células T CD4 +, células T CD8 +, células B y células asesinas naturales). Usando este método, las células magnéticas y marcadas con fluorescencia pueden ser purificadas y analizadas.
