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Scheiding van immuuncelsubpopulaties in perifere bloedmonsters van kinderen met infectieuze mononucleosis
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Scheiding van immuuncelsubpopulaties in perifere bloedmonsters van kinderen met infectieuze mononucleosis
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08:44 min
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September 07, 2022
DOI:
1Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children’s Hospital,Capital Medical University, National Center for Children’s Health, 2Research Unit of Critical Infection in Children, 2019RU016,Chinese Academy of Medical Sciences
Summary
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We beschrijven een methode die immunomagnetische kralen en fluorescentie-geactiveerde celsortering combineert om gedefinieerde immuuncelsubpopulaties van perifere mononucleaire bloedcellen (monocyten, CD4 + T-cellen, CD8 + T-cellen, B-cellen en natural killer-cellen) te isoleren en te analyseren. Met behulp van deze methode kunnen magnetische en fluorescerend gelabelde cellen worden gezuiverd en geanalyseerd.
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Cite this Article
Zhang, L., Liu, M., Zhang, M., Ai, J., Tian, J., Wang, R., Xie, Z. Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis. J. Vis. Exp. (187), e64212, doi:10.3791/64212 (2022).
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