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Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C
 

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Article DOI: 10.3791/64166-v • 09:32 min • October 14th, 2022
October 14th, 2022 •
1, 1, 2, 1, 3, 4, 1, 1,5
1EMBL: European Molecular Biology Laboratory, 2Institut Curie, 3Agilent Technologies, 4Genmab BV, 5Collège de France

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This protocol describes the Capture Hi-C method used to characterize the 3D organization of megabased-sized targeted genomic regions at high-resolution, including boundaries of topologically associating domains (TADs) and long-range chromatin interactions between regulatory and other DNA sequence elements.

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Capture Hi-C High-resolution 3D Chromatin Organization Allele Specificity Time Effectiveness Affordability Model System Cell Types Developmentally Regulated Chromatin Landscapes Genome Topology Gene Regulation Tryphtonise Count Cells Automated Cell Counter Viability Staining Crosslinking Fixation Solution Glycine PBS
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