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达乌尔蝴蝶幼虫的大规模趋重试验

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JoVE Journal Comportamento

Large-Scale Gravitaxis Assay of Caenorhabditis Dauer Larvae

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达乌尔蝴蝶幼虫的大规模趋重试验

DOI:

10.3791/64062-v

•

07:53 min

•

May 31, 2022

•

Caroline Ackley, Lindsey Washiashi, Ruchira Krishnamurthy, Joel H. Rothman

¹Molecular Cellular and Developmental Biology,University of California

Capítulos

00:05Introduction
00:47Chamber Preparation
01:37Filling the Chambers with Agar
02:33Isolating Dauer Larvae
04:01Adding Dauers to the Chamber
05:03Running and Scoring the Assay
06:38Results: Comparison of Gravitaxis Behavior Across Species
07:14Conclusion

Summary

Tadução automática

本协议概述了用 Caenorhabditis dauer幼虫进行大规模重力测定的方法。与基于平板的测定相比，该协议可以更好地检测重症行为。

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Keywords: Gravitaxis Assay Caenorhabditis Dauer Larvae High Throughput Gravity Sensation Vestibular System NGM Agar Serological Pipettes Bunsen Burner Razor Blade Pliers Tweezers Plastic Cutting Surface Fume Hood OP50 Bacteria M9 Buffer

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