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Sciences du comportement
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Large-Scale Gravitaxis Assay of Caenorhabditis Dauer Larvae
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Sciences du comportement
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Journal JoVE Sciences du comportement
Large-Scale Gravitaxis Assay of Caenorhabditis Dauer Larvae

Large-Scale Gravitaxis Assay of Caenorhabditis Dauer Larvae

DOI:
10.3791/64062-v

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07:53 min

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May 31, 2022

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, , ,
1Molecular Cellular and Developmental Biology,University of California

Chapitres

  • 00:05Introduction
  • 00:47Chamber Preparation
  • 01:37Filling the Chambers with Agar
  • 02:33Isolating Dauer Larvae
  • 04:01Adding Dauers to the Chamber
  • 05:03Running and Scoring the Assay
  • 06:38Results: Comparison of Gravitaxis Behavior Across Species
  • 07:14Conclusion

Summary

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The present protocol outlines methods for conducting a large-scale gravitaxis assay with Caenorhabditis dauer larvae. This protocol allows for better detection of gravitaxis behavior compared with a plate-based assay.

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Gravitaxis AssayCaenorhabditis Dauer LarvaeHigh ThroughputGravity SensationVestibular SystemNGM AgarSerological PipettesBunsen BurnerRazor BladePliersTweezersPlastic Cutting SurfaceFume HoodOP50 BacteriaM9 Buffer

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