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制备与DNA修复因子络合的核小体核心颗粒用于冷冻电镜结构测定
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Bioquímica
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JoVE Journal Bioquímica
Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination
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制备与DNA修复因子络合的核小体核心颗粒用于冷冻电镜结构测定

DOI:
10.3791/64061-v

•

07:59 min

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August 17, 2022

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, , , ,
1Genome Integrity and Structural Biology Laboratory,National Institute of Environmental Health Sciences, National Institutes of Health

Capítulos

  • 00:04Introduction
  • 00:58Preparation of NCP-DNA Repair Factor Complex
  • 02:31Freezing Nucleosomal Complex
  • 04:25Screen Grids
  • 06:02Results: Preparation of Nucleosomal Complexes Using Frozen TEM Grids
  • 07:20Conclusion

Summary

Tadução automática

Tadução automática

该协议详细概述了使用两种用于冷冻TEM网格的样品制备方法制备核小体复合物。

Tags

NucleosomeDNA RepairCryo-electron MicroscopySample PreparationCross-linkingSize Exclusion ChromatographyBuffer ExchangeProtein Complex

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