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توصيف البروتين عبر الغشاء الحويصلة خارج الخلية بواسطة قياس التدفق الخلوي النانوي
JoVE Journal
Biologia
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JoVE Journal Biologia
Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry
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توصيف البروتين عبر الغشاء الحويصلة خارج الخلية بواسطة قياس التدفق الخلوي النانوي

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12:27 min

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July 26, 2022

DOI:

10.3791/64020-v

DOI:
10.3791/64020-v

•

12:27 min
July 26, 2022

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6 Views
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1NanoFCM Co., Ltd, 2School of Sport, Exercise and Health Sciences,Loughborough University, 3Biomolecular Sciences Research Centre,Sheffield Hallam University

Tags

Single Extracellular VesicleTransmembrane ProteinNano-flow CytometryEV CharacterizationParticle AnalysisLabel-free DetectionFluorescence DetectionVesicle SubpopulationsPlasmaCSFCell CultureVirusesLipid NanoparticlesNanomaterialsLabeling ProtocolQC BeadsPressure ControlSample DilutionCleaning SolutionSize Standard Beads

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Lees, R., Tempest, R., Law, A., Aubert, D., Davies, O. G., Williams, S., Peake, N., Peacock, B. Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry. J. Vis. Exp. (185), e64020, doi:10.3791/64020 (2022).

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