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Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry
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July 26, 2022
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The latest generation of EV characterization tools are capable of single EV analysis across multiple parameters simultaneously. Nano-flow cytometry measures all biological particles larger than 45 nm without labeling and identifies specific characteristics of subpopulations by a variety of fluorescent labeling techniques.
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Single Extracellular VesicleTransmembrane ProteinNano-flow CytometryEV CharacterizationParticle AnalysisLabel-free DetectionFluorescence DetectionVesicle SubpopulationsPlasmaCSFCell CultureVirusesLipid NanoparticlesNanomaterialsLabeling ProtocolQC BeadsPressure ControlSample DilutionCleaning SolutionSize Standard Beads
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Lees, R., Tempest, R., Law, A., Aubert, D., Davies, O. G., Williams, S., Peake, N., Peacock, B. Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry. J. Vis. Exp. (185), e64020, doi:10.3791/64020 (2022).
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