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研究细胞内囊泡破裂功能的光动力方法

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

A Photodynamic Approach to Study Function of Intracellular Vesicle Rupture

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研究细胞内囊泡破裂功能的光动力方法

DOI:

10.3791/63962-v

•

05:16 min

•

March 17, 2023

•

Yu-Hsien Hung, Hsiu-Jung Wang, Jen-Shuang Wang, Chia-Hao Hsu, Huan-Yuan Chen

¹Inflammation Core Facility, Institute of Biomedical Sciences,Academia Sinica

Capítulos

00:04Introduction
00:44Transfection and AlPcS_2a Staining
03:00Sample Imaging and Light Illumination
03:47Results: AlPcS_2a-Mediated Chromophore‐Assisted Laser Inactivation for Studying patiotemporal Damage of Intracellular Vesicles in Live Cells
04:25Conclusion

Summary

Tadução automática

AlPcS_2a介导的发色团辅助激光灭活（CALI）是研究活细胞中细胞内囊泡（IVs）时空损伤的强大工具。

Tags

Keywords: Photodynamic Approach Intracellular Vesicle Rupture Endocytosis Signal Pathways Chromophore-assisted Laser Inactivation Subcellular Damage HeLa Cells Gal3-GFP AlPcS2a Lysosome Confocal Microscopy

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