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使用质谱法表征单个神经元中的RNA修饰
JoVE Journal
Química
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JoVE Journal Química
Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry
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使用质谱法表征单个神经元中的RNA修饰

1,975 Views

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08:45 min

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April 21, 2022

DOI:

10.3791/63940-v

DOI:
10.3791/63940-v

•

08:45 min
April 21, 2022

•

1 Views
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1Beckman Institute for Advanced Science and Technology,University of Illinois Urbana-Champaign, 2Department of Chemistry,University of Illinois Urbana-Champaign

Tags

RNA ModificationsSingle NeuronsMass SpectrometryPost-transcriptional RegulationAplysia CalifornicaGanglia IsolationProtease TreatmentNeuron IsolationArtificial SeawaterMass Spectrometry Analysis

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Clark, K. D., Rubakhin, S. S., Sweedler, J. V. Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry. J. Vis. Exp. (182), e63940, doi:10.3791/63940 (2022).

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