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Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry
JoVE 杂志
化学
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JoVE 杂志 化学
Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

2,064 Views

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08:45 min

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April 21, 2022

DOI:

10.3791/63940-v

DOI:
10.3791/63940-v

•

08:45 min
April 21, 2022

•

7 Views
, ,
1Beckman Institute for Advanced Science and Technology,University of Illinois Urbana-Champaign, 2Department of Chemistry,University of Illinois Urbana-Champaign

Summary

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Post-transcriptional modifications of RNA represent an understudied layer of translation regulation that has recently been linked to central nervous system plasticity. Here, sample preparation and liquid chromatography-tandem mass spectrometry approach is described for simultaneous characterization of numerous RNA modifications in single neurons.

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Cite this Article

Clark, K. D., Rubakhin, S. S., Sweedler, J. V. Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry. J. Vis. Exp. (182), e63940, doi:10.3791/63940 (2022).

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