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Developing Drosophila melanogaster Models for Imaging and Optogenetic Control of Cardiac Function
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Bioengenharia
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JoVE Journal Bioengenharia
Developing Drosophila melanogaster Models for Imaging and Optogenetic Control of Cardiac Function

Developing Drosophila melanogaster Models for Imaging and Optogenetic Control of Cardiac Function

DOI:
10.3791/63939-v

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08:43 min

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August 25, 2022

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, , , , ,
1Department of Biomedical Engineering,Washington University in St. Louis, 2Department of Computer Science and Engineering,Washington University in St. Louis

Capítulos

  • 00:04Introduction
  • 00:57Drosophila Genetic Crosses and Media Preparation
  • 02:30Optogenetic Control of Drosophila Heart
  • 05:26Image Analysis
  • 06:37Results: Drosophila OCT Imaging and Heart Beating Modulation
  • 08:08Conclusion

Summary

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The present protocol describes the generation of Drosophila melanogaster expressing eNpHR2.0 or ReaChR opsins in the heart for OCT imaging and optogenetic heart pacing. Detailed instructions for Drosophila OCT imaging and heart beating modulation, including the simulation of restorable heart arrest, bradycardia, and tachycardia in live animals at different developmental stages, are reported.

Tags

Keywords: Drosophila MelanogasterOptogenetic ControlCardiac FunctionOCT ImagingOptogenetic PacingHeart Disease ModelsNon-invasive ImagingHeart OrganoidsZebrafishEmbryonic Heart

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