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Developing Drosophila melanogaster Models for Imaging and Optogenetic Control of Cardiac Function
JoVE 杂志
生物工程
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JoVE 杂志 生物工程
Developing Drosophila melanogaster Models for Imaging and Optogenetic Control of Cardiac Function

Developing Drosophila melanogaster Models for Imaging and Optogenetic Control of Cardiac Function

2,250 Views

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08:43 min

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August 25, 2022

DOI:

10.3791/63939-v

DOI:
10.3791/63939-v

•

08:43 min
August 25, 2022

•

6 Views
, , , , ,
1Department of Biomedical Engineering,Washington University in St. Louis, 2Department of Computer Science and Engineering,Washington University in St. Louis

Summary

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The present protocol describes the generation of Drosophila melanogaster expressing eNpHR2.0 or ReaChR opsins in the heart for OCT imaging and optogenetic heart pacing. Detailed instructions for Drosophila OCT imaging and heart beating modulation, including the simulation of restorable heart arrest, bradycardia, and tachycardia in live animals at different developmental stages, are reported.

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Cite this Article

Gracheva, E., Wang, F., Matt, A., Liang, H., Fishman, M., Zhou, C. Developing Drosophila melanogaster Models for Imaging and Optogenetic Control of Cardiac Function. J. Vis. Exp. (186), e63939, doi:10.3791/63939 (2022).

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