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液体および氷中のウイルス集合体の高解像度イメージングの進歩
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08:31 min
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July 20, 2022
DOI:
1Department of Biomedical Engineering,Pennsylvania State University, 2Department of Materials Science and Engineering,McMaster University, 3Bioinformatics and Genomics Graduate Program, Huck Institutes of the Life Sciences,Pennsylvania State University, 4Molecular, Cellular, and Integrative Biosciences Graduate Program, Huck Institutes of the Life Sciences,Pennsylvania State University, 5Materials Research Institute,Pennsylvania State University, 6Huck Institutes of the Life Sciences,Pennsylvania State University, 7Applications team,Direct Electron, 8Application Scientist,Protochips, Inc., 9Department of Biology,Wake Forest University
Summary
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ここでは、透過型電子顕微鏡を使用してナノスケールでの液体EMおよびクライオEM分析に適したウイルスアセンブリを調製するためのプロトコルについて説明します。
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Cite this Article
DiCecco, L., Berry, S., Jonaid, G. M., Solares, M. J., Kaylor, L., Gray, J. L., Bator, C., Dearnaley, W. J., Spilman, M., Dressel-Dukes, M. J., Grandfield, K., McDonald Esstman, S. M., Kelly, D. F. Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice. J. Vis. Exp. (185), e63856, doi:10.3791/63856 (2022).
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