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Preparation of Rat Sciatic Nerve for Ex Vivo Neurophysiology
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JoVE Journal Neurociência
Preparation of Rat Sciatic Nerve for Ex Vivo Neurophysiology

Preparation of Rat Sciatic Nerve for Ex Vivo Neurophysiology

DOI:
10.3791/63838-v

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09:09 min

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July 12, 2022

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, , , , ,
1Department of Electrical and Electronic Engineering,Imperial College London, 2Centre for Bioinspired Technology,Imperial College London, 3Care Research and Technology (CR&T),Imperial College London and the University of Surrey, 4Dementia Institute (UK DRI), 5Department of Bioengineering,Imperial College London

Capítulos

  • 00:04Introduction
  • 00:38Dissection Protocol
  • 02:16Nerve Cleaning Procedure
  • 03:21Equipment Setup
  • 05:28Electrode Implantation on the Nerve in the Bath
  • 06:20Stimulation and Recording
  • 07:31Results: Ex Vivo Neurophysiology of Rat Whole Sciatic Nerve Tissue
  • 08:19Conclusion

Summary

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This protocol describes the preparation of rat whole sciatic nerve tissue for ex vivo electrophysiological stimulation and recording in an environmentally-regulated, two-compartment, perfused saline bath.

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Keywords: RatSciatic NerveEx Vivo NeurophysiologyNerve BlockHigh Frequency/high Amplitude CurrentIn Vivo ElectrophysiologyEuthanizationDissectionKrebs-Henseleit BufferMKHBHemostatsSpinal CleftForcepsScissorsPetri DishSutures

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