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Hippocampal Neuronal Cultures to Detect and Study New Pathogenic Antibodies Involved in Autoimmune Encephalitis
JoVE Journal
Neurociência
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JoVE Journal Neurociência
Hippocampal Neuronal Cultures to Detect and Study New Pathogenic Antibodies Involved in Autoimmune Encephalitis

Hippocampal Neuronal Cultures to Detect and Study New Pathogenic Antibodies Involved in Autoimmune Encephalitis

DOI:
10.3791/63829-v

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08:20 min

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June 02, 2022

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, , ,
1ICFO-Institut de Ciències Fotòniques,The Barcelona Institute of Science and Technology, 2Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Hospital Clínic,Universitat de Barcelona

Capítulos

  • 00:05Introduction
  • 00:55Hippocampal Isolation
  • 02:14Cell Dissociation
  • 03:44Live Fluorescent Immunostaining
  • 04:34Calcium Imaging
  • 05:57Results: Determination of the Presence of Antibodies in the Patient’s Sample with Immunostaining
  • 07:19Conclusion

Summary

Tadução automática

Tadução automática

Autoimmune encephalitis is a new category of antibody-mediated diseases of the central nervous system. Hippocampal neurons can be used to discover and characterize these antibodies. This article provides a protocol for primary cell culture and immunostaining to determine autoantibodies in the serum and cerebrospinal fluid of patients.

Tags

Keywords: Hippocampal Neuronal CulturesAutoimmune EncephalitisPathogenic AntibodiesSurface AntigensIntracellular AntigensEmbryonic Brain DissectionHippocampus IsolationEnzymatic DissociationCell Culture

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