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从人包皮中分离、培养和表征原代施旺细胞、角质形成细胞和成纤维细胞

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Isolation, Culture, and Characterization of Primary Schwann Cells, Keratinocytes, and Fibroblasts from Human Foreskin

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从人包皮中分离、培养和表征原代施旺细胞、角质形成细胞和成纤维细胞

DOI:

10.3791/63776-v

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10:36 min

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March 23, 2022

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Mashanipalya G. Jagadeeshaprasad, Prem Kumar Govindappa, Amanda M. Nelson, John C. Elfar

¹Department of Orthopaedics and Rehabilitation, Center for Orthopaedic Research and Translational Science (CORTS),The Pennsylvania State University College of Medicine, ²Department of Dermatology,The Pennsylvania State University College of Medicine

Capítulos

00:04Introduction
01:17Epidermis and Dermis Separation Procedure
03:03Keratinocyte Isolation from the Epidermis
05:02Isolation of Schwann Cells (SCs) and Fibroblast from the Dermis
08:18Results: Characterization of Human Foreskin-Derived Primary Keratinocytes, Schwann Cells, and Fibroblasts
09:43Conclusion

Summary

Tadução automática

研究与肌肉骨骼损伤相关的伤口愈合通常需要评估施万细胞（SCs）、角质形成细胞和成纤维细胞之间的体外相互作用。该协议描述了从人包皮中分离，培养和表征这些原代细胞。

Tags

Keywords: Primary Schwann Cells Keratinocytes Fibroblasts Human Foreskin Skin Cells Cell Isolation Cell Culture Cell Interactions Skin Homeostasis Skin Pathophysiology Wound Healing Skin Disease

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