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Isolation, Culture, and Characterization of Primary Schwann Cells, Keratinocytes, and Fibroblasts from Human Foreskin
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JoVE Journal 神経科学
Isolation, Culture, and Characterization of Primary Schwann Cells, Keratinocytes, and Fibroblasts from Human Foreskin

Isolation, Culture, and Characterization of Primary Schwann Cells, Keratinocytes, and Fibroblasts from Human Foreskin

5,057 Views

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10:36 min

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March 23, 2022

DOI:

10.3791/63776-v

DOI:
10.3791/63776-v

•

10:36 min
March 23, 2022

•

29 Views
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1Department of Orthopaedics and Rehabilitation, Center for Orthopaedic Research and Translational Science (CORTS),The Pennsylvania State University College of Medicine, 2皮膚学部,The Pennsylvania State University College of Medicine

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The study of wound healing associated with musculoskeletal injury often requires the assessment of in vitro interactions between Schwann cells (SCs), keratinocytes, and fibroblasts. This protocol describes the isolation, culturing, and characterization of these primary cells from the human foreskin.

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Primary Schwann CellsKeratinocytesFibroblastsHuman ForeskinSkin CellsCell IsolationCell CultureCell InteractionsSkin HomeostasisSkin PathophysiologyWound HealingSkin Disease

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Jagadeeshaprasad, M. G., Govindappa, P. K., Nelson, A. M., Elfar, J. C. Isolation, Culture, and Characterization of Primary Schwann Cells, Keratinocytes, and Fibroblasts from Human Foreskin. J. Vis. Exp. (181), e63776, doi:10.3791/63776 (2022).

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