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Biologia do Desenvolvimento

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זיהוי וכימות של תאים שומרי תוויות בחותכות עכבר באמצעות גישת שחזור תלת-ממדית לאחר ניקוי רקמות

JoVE Journal
Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Detection and Quantitation of Label-Retaining Cells in Mouse Incisors using a 3D Reconstruction Approach after Tissue Clearing

Please note that all translations are automatically generated. Click here for the English version.

זיהוי וכימות של תאים שומרי תוויות בחותכות עכבר באמצעות גישת שחזור תלת-ממדית לאחר ניקוי רקמות

1,677 Views

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10:32 min

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June 10, 2022

DOI:

10.3791/63721-v

DOI:

10.3791/63721-v

•

10:32 min

June 10, 2022

•

8 Views

Changchun Dong*¹, Bikash Lamichhane*¹, Yongxu Zhang, Xiaofang Wang

¹Department of Biomedical Sciences,Texas A&M School of Dentistry

Tags

3D Reconstruction Label-retaining Cells Mouse Incisor Stem Cell Niche 5-ethynyl-2′-deoxyuridine (EdU) Staining Whole Mount Decalcification Decolorization De-lipidation Tert-butanol-polyethylene Glycol (tB-PEG)Benzyl Benzoate-polyethylene Glycol (BB-PEG) Clearing Confocal Imaging

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