Journal
/
Biologia do Desenvolvimento
/
Påvisning og kvantificering af etiketbevarende celler i musefortænder ved hjælp af en 3D-rekonstruktionstilgang efter vævsrydning
JoVE Journal
Biologia do Desenvolvimento
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia do Desenvolvimento
Detection and Quantitation of Label-Retaining Cells in Mouse Incisors using a 3D Reconstruction Approach after Tissue Clearing

Påvisning og kvantificering af etiketbevarende celler i musefortænder ved hjælp af en 3D-rekonstruktionstilgang efter vævsrydning

1,677 Views

•

10:32 min

•

June 10, 2022

DOI:

10.3791/63721-v

DOI:
10.3791/63721-v

•

10:32 min
June 10, 2022

•

8 Views
, , ,
1Department of Biomedical Sciences,Texas A&M School of Dentistry

Tags

3D ReconstructionLabel-retaining CellsMouse IncisorStem Cell Niche5-ethynyl-2′-deoxyuridine (EdU) StainingWhole MountDecalcificationDecolorizationDe-lipidationTert-butanol-polyethylene Glycol (tB-PEG)Benzyl Benzoate-polyethylene Glycol (BB-PEG) ClearingConfocal Imaging

Read Article

Cite this Article

Dong, C., Lamichhane, B., Zhang, Y., Wang, X. Detection and Quantitation of Label-Retaining Cells in Mouse Incisors using a 3D Reconstruction Approach after Tissue Clearing. J. Vis. Exp. (184), e63721, doi:10.3791/63721 (2022).

Download .ris file

Copy

Share Video

.

Copy