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Biologia do Desenvolvimento
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Detection and Quantitation of Label-Retaining Cells in Mouse Incisors using a 3D Reconstruction Approach after Tissue Clearing
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Detection and Quantitation of Label-Retaining Cells in Mouse Incisors using a 3D Reconstruction Approach after Tissue Clearing

Detection and Quantitation of Label-Retaining Cells in Mouse Incisors using a 3D Reconstruction Approach after Tissue Clearing

DOI:
10.3791/63721-v

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10:32 min

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June 10, 2022

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, , ,
1Department of Biomedical Sciences,Texas A&M School of Dentistry

Capítulos

  • 00:05Introduction
  • 00:42Tissue Clearing of Mandible Using PEGASOS Technique
  • 03:03Confocal Imaging of Tissue-Cleared Mandible
  • 04:37Image Processing, 3D Image Reconstruction, and Quantification of Label-Retaining Cells
  • 08:25Results: Detection and Quantitation of Label-Retaining Cells in Mouse Incisors
  • 09:50Conclusion

Summary

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The mouse incisor contains valuable label-retaining cells in its stem cell niche. We have a novel way to unbiasedly detect and quantify the label-retaining cells; our study used EdU labeling and a 3D reconstruction approach after PEGASOS tissue clearing of the mandible.

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Keywords: 3D ReconstructionLabel-retaining CellsMouse IncisorStem Cell Niche5-ethynyl-2′-deoxyuridine (EdU) StainingWhole MountDecalcificationDecolorizationDe-lipidationTert-butanol-polyethylene Glycol (tB-PEG)Benzyl Benzoate-polyethylene Glycol (BB-PEG) ClearingConfocal Imaging

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