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基于宽带受激拉曼散射显微镜的多重化学成像

JoVE Journal
Engenharia

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JoVE Journal Engenharia

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

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基于宽带受激拉曼散射显微镜的多重化学成像

DOI:

10.3791/63709-v

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09:57 min

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July 25, 2022

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Alejandro De la Cadena, Federico Vernuccio, Benedetta Talone, Arianna Bresci, Chiara Ceconello, Subir Das, Renzo Vanna, Giulio Cerullo², Dario Polli²

¹Dipartimento di Fisica,Politecnico di Milano, ²Institute for Photonics and Nanotechnologies (IFN-CNR)

Capítulos

00:04Introduction
01:17Sample Preparation
02:22Optimizing Pump and Stokes Beams
05:54Setting Up the Spectral Detection for SRS Imaging
07:07Chemometrics of Hyperspectral SRS Data
07:54Results: RIN Spectra and the Effect of Balanced Detection
09:15Conclusion

Summary

Tadução automática

我们提出了一种使用宽带受激拉曼散射（SRS）显微镜获取化学图像的方案。基于与差分多通道锁相检测一起工作的SRS显微镜，该协议描述了样品制备，SRS设备的调整以及化学计量学以解开化学非均质样品的不同成分。

Tags

Stimulated Raman Scattering Microscopy Broadband Vibrational Spectrum Hyperspectral Imaging Label-free Non-invasive PMMA Microbeads Polystyrene Microbeads Dimethyl Sulfoxide Olive Oil Optical Parametric Oscillator Prism Compressor Dispersion Compensation

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