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प्रोटीन फॉस्फोराइलेशन की मात्रा का ठहराव के लिए एक अनुकूलित एकल अणु पुल-डाउन परख

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation

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प्रोटीन फॉस्फोराइलेशन की मात्रा का ठहराव के लिए एक अनुकूलित एकल अणु पुल-डाउन परख

2,553 Views

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07:45 min

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June 06, 2022

DOI:

10.3791/63665-v

DOI:

10.3791/63665-v

•

07:45 min

June 06, 2022

•

7 Views

Elizabeth M. Bailey, Emanuel Salazar-Cavazos, Rachel M. Grattan, Michael J. Wester, David J. Schodt, Julian A. Rojo, Keith A. Lidke³, Diane S. Lidke³

¹Department of Pathology,University of New Mexico School of Medicine, ²Department of Physics and Astronomy,University of New Mexico, ³Comprehensive Cancer Center,University of New Mexico Health Sciences Center

Tags

AI-powered Protein Phosphorylation Quantification Single-molecule Pull-down Assay SiMPull Protocol Antibody Labeling Autofluorescence Reduction Single-molecule Analysis Phosphorylation Status Of Individual Proteins Alternative To Western Blotting And Mass Spectrometry

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