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Capítulos
- 00:05Introduction
- 00:43Plasmid Transfection
- 02:16Cell Collection
- 02:52Purification of Ubiquitinated Proteins under Nondenaturing Conditions
- 05:01Purification of Ubiquitinated Proteins under Denaturing Conditions
- 07:49Detection of Purified Ubiquitinated Proteins by Western Blotting
- 08:29Results: Ubiquitinated p53 Proteins Purified under Nondenaturing and Denaturing Conditions
- 10:22Conclusion
Das Protokoll beschreibt eine Schritt-für-Schritt-Methode zur Reinigung ubiquitinierter Proteine aus Säugetierzellen am Beispiel des Tumorsuppressorproteins p53. Ubiquitinierte p53-Proteine wurden unter strengen nichtdenaturierenden und denaturierenden Bedingungen aus Zellen gereinigt.
