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Purification of Ubiquitinated p53 Proteins from Mammalian Cells
JoVE Journal
Biochimica
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JoVE Journal Biochimica
Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

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10:55 min

March 21, 2022

DOI:

10:55 min
March 21, 2022

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Summary

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The protocol describes a step-by-step method to purify ubiquitinated proteins from mammalian cells using the p53 tumor suppressor protein as an example. Ubiquitinated p53 proteins were purified from cells under stringent nondenaturing and denaturing conditions.

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