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Imaging Neutrophil Migration in the Mouse Skin to Investigate Subcellular Membrane Remodeling Under Physiological Conditions
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Biologia
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JoVE Journal Biologia
Imaging Neutrophil Migration in the Mouse Skin to Investigate Subcellular Membrane Remodeling Under Physiological Conditions

Imaging Neutrophil Migration in the Mouse Skin to Investigate Subcellular Membrane Remodeling Under Physiological Conditions

1,176 Views

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08:41 min

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May 10, 2022

DOI:

10.3791/63581-v

DOI:
10.3791/63581-v

•

08:41 min
May 10, 2022

•

1 Views
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1Laboratory of Cellular and Molecular Biology, Center for Cancer Research,National Cancer Institute, 2Lineberger Comprehensive Cancer Center,UNC-Chapel Hill

Summary

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Neutrophil migration relies on the rapid and continuous remodeling of the plasma membrane in response to chemoattractant and its interactions with the extracellular microenvironment. Described herein is a procedure based on Intravital Subcellular Microscopy to investigate the dynamic of membrane remodeling in neutrophils injected in the ear of anesthetized mice.

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Cite this Article

Melis, N., Subramanian, B., Chen, D., Weigert, R. Imaging Neutrophil Migration in the Mouse Skin to Investigate Subcellular Membrane Remodeling Under Physiological Conditions. J. Vis. Exp. (183), e63581, doi:10.3791/63581 (2022).

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