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1Oxidative Stress Group, Dept. Cell Biology, Microbiology and Molecular Biology, College of Arts and Sciences,University of South Florida, 2Cepheid (Danaher Corp.),US Technical Operations, 3Department of Clinical Laboratory Sciences, College of Applied Medical Sciences,King Saud University, 4Department of Chemistry and Biochemistry, Institute of Environment,Florida International University, 5Leicester School of Allied Health Sciences, Faculty of Health and Life Sciences,De Montfort University
Capítulos
- 00:05Introduction
- 01:10Cell Lysis
- 02:56Electrophoresis
- 04:42Propidium Iodide Staining
- 05:43Results: Analyzing DNA Damage Using High Throughput Alkaline Comet Assay
- 07:17Conclusion
Kometanalysen er et populært middel til at opdage DNA-skader. Denne undersøgelse beskriver en tilgang til at køre dias i repræsentative varianter af kometanalysen. Denne tilgang øgede antallet af prøver betydeligt, samtidig med at analysens driftstid blev reduceret, antallet af glidemanipulationer og risikoen for skade på geler.
