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A High-Throughput Comet Assay Approach for Assessing Cellular DNA Damage
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May 10, 2022
DOI:
1Oxidative Stress Group, Dept. Cell Biology, Microbiology and Molecular Biology, College of Arts and Sciences,University of South Florida, 2Cepheid (Danaher Corp.),US Technical Operations, 3Department of Clinical Laboratory Sciences, College of Applied Medical Sciences,King Saud University, 4Department of Chemistry and Biochemistry, Institute of Environment,Florida International University, 5Leicester School of Allied Health Sciences, Faculty of Health and Life Sciences,De Montfort University
Summary
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The comet assay is a popular means of detecting DNA damage. This study describes an approach to running slides in representative variants of the comet assay. This approach significantly increased the number of samples while decreasing assay run-time, the number of slide manipulations, and the risk of damage to gels.
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Ji, Y., Karbaschi, M., Abdulwahed, A., Quinete, N. S., Evans, M. D., Cooke, M. S. A High-Throughput Comet Assay Approach for Assessing Cellular DNA Damage. J. Vis. Exp. (183), e63559, doi:10.3791/63559 (2022).
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