Journal
/
Bioquímica
/
福斯特共振能量转移映射：阐明全球结构特征的新方法
JoVE Journal
Bioquímica
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Bioquímica
Förster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features
Please note that all translations are automatically generated. Click here for the English version.

福斯特共振能量转移映射：阐明全球结构特征的新方法

2,300 Views

•

07:09 min

•

March 16, 2022

DOI:

10.3791/63433-v

DOI:
10.3791/63433-v

•

07:09 min
March 16, 2022

•

2 Views
, ,
1Molecular Biology and Biochemistry Department,Wesleyan University, 2Molecular Biophysics Program,Wesleyan University

Summary

Automatically generated

该研究详细介绍了FRET映射的方法，包括标记位点的选择，染料的选择，采集和数据分析。该方法可有效确定蛋白质系统中的结合位点、构象变化和动态运动，如果与现有的3-D结构信息结合使用，这种方法最有用。

Explore More Videos

Keywords Extracted From The Given Text:FRET MappingLigand Binding SitesSubunit OrientationConformational ChangesDynamic MotionsBiomolecular SystemLabeling SitesR0 ValuesDonor DyeAcceptor DyeSpectrofluorometerEmission ScanExcitation Wavelength

Read Article

Cite this Article

Northrop, J., Oliver, D. B., Mukerji, I. Förster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features. J. Vis. Exp. (181), e63433, doi:10.3791/63433 (2022).

Download .ris file

Copy

Share Video

.

Copy