/
/
Förster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Förster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features
2,300 Views
•
07:09 min
•
March 16, 2022
DOI:
Summary
Automatically generated
The study details the methodology of FRET mapping including the selection of labeling sites, choice of dyes, acquisition, and data analysis. This methodology is effective at determining binding sites, conformational changes, and dynamic motions in protein systems and is most useful if performed in conjunction with existing 3-D structural information.
Read Article
Cite this Article
Northrop, J., Oliver, D. B., Mukerji, I. Förster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features. J. Vis. Exp. (181), e63433, doi:10.3791/63433 (2022).
Copy