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クライオスパーク、リオン、サイピオンによる堅牢な単一粒子クライオ電子顕微鏡(クライオEM)処理ワークフロー
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Bioquímica
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JoVE Journal Bioquímica
A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion
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クライオスパーク、リオン、サイピオンによる堅牢な単一粒子クライオ電子顕微鏡(クライオEM)処理ワークフロー

10,521 Views

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13:43 min

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January 31, 2022

DOI:

10.3791/63387-v

DOI:
10.3791/63387-v

•

13:43 min
January 31, 2022

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25 Views
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1Institute for Quantitative Biomedicine,Rutgers University, 2Department of Biochemistry & Microbiology,Rutgers University

Tags

Cryo-EMSingle-particle AnalysisCryoSPARCRELIONScipionAdeno-associated VirusGene TherapyPatch Motion CorrectionCTF EstimationMicrograph CurationManual Particle PickingTemplate GenerationParticle Extraction

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DiIorio, M. C., Kulczyk, A. W. A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion. J. Vis. Exp. (179), e63387, doi:10.3791/63387 (2022).

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